Quantitative PCR (qPCR also known as real-time PCR) simultaneously amplifies and detects targeted DNA allowing scientists to discover the starting amount of a specific DNA sequence in an experimental sample. This powerful and precise technology has become a cornerstone of modern genetics research, biological monitoring, and medical diagnostics. For students, performing qPCR offers the challenge of performing a mathematically and technically advanced experiment, the opportunity to understand key molecular concepts through hands-on learning, and an essential skill for future research work. In this specially adapted education qPCR experiment, students will quantify the DNA concentration of four experimental samples using a standard curve approach and then confirm the experiment’s specificity and accuracy through gel electrophoresis, melt curve analysis, and data analysis.
* Requires wet ice shipment
**This kit must be used with a RT Thermal Cycler**
Discovering Quantitative PCR Amplification and Analysis
Quantitative PCR (qPCR also known as real-time PCR) simultaneously amplifies and detects targeted DNA allowing scientists to discover the starting amount of a specific DNA sequence in an experimental sample. This powerful and precise technology has become a cornerstone of modern genetics research, biological monitoring, and medical diagnostics. For students, performing qPCR offers the challenge of performing a mathematically and technically advanced experiment, the opportunity to understand key molecular concepts through hands-on learning, and an essential skill for future research work. In this specially adapted education qPCR experiment, students will quantify the DNA concentration of four experimental samples using a standard curve approach and then confirm the experiment’s specificity and accuracy through gel electrophoresis, melt curve analysis, and data analysis.
* Requires wet ice shipment
**This kit must be used with a RT Thermal Cycler**
Description
QPCR – 90 minutes
Single Amplification Confirmation by Electrophoresis – 60 minutes
Staining – 5 minutes to overnight
Analysis of Results 45 mins
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