In this experiment, students will conduct a DNA fingerprinting exercise on simulated patient samples to determine if family members are heterozygous or homozygous for Huntington’s Disease. Students will then analyze the amplified DNA segments by agarose gel electrophoresis.
Instructions, PCR EdvoBeads™, LyphoPrimer™ and LyphoTemplate™ DNA, DNA samples, TE buffer, UltraSpec-Agarose™, electrophoresis buffer (50x), SYBR® Safe stain, FlashBlue™ liquid stain, microcentifuge tubes, 0.2 ml PCR tubes, and wax beads.
All You Need:
Thermal cycler, horizontal gel electrophoresis apparatus, power supply, microcentrifuge, UV transilluminator or UV photodocumentation system, UV safety goggles, white light visualization system (optional if staining with FlashBlue™), automatic micropipettes and tips, microwave or hot plate, 250 ml flasks or beakers, hot gloves, and disposable lab gloves.
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Diagnosing Huntington’s Disease Using PCR
In this experiment, students will conduct a DNA fingerprinting exercise on simulated patient samples to determine if family members are heterozygous or homozygous for Huntington’s Disease. Students will then analyze the amplified DNA segments by agarose gel electrophoresis.
Description
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