Water Quality Testing III: Multiplex PCR Testing of Water Contaminants
Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, Water Bath, Microcentrifuge, Microwave or Hot plate, & Isopropanol.
Storage:
Some Components Require Refrigerator or Freezer Storage Upon Receipt
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Water Quality Testing III: Multiplex PCR Testing of Water Contaminants
Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
Description
PCR Setup – 10 minutes
PCR – 2 hours
Electrophoresis – 60 minutes
Staining – 5 mins or overnight
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