The Mother of All Experiments: Exploring Human Origin by PCR Amplification of Mitochondrial DNA
In this experiment, students will isolate their mitochondrial DNA and use the Polymerase Chain Reaction (PCR) to amplify two separate regions of the mitochondrial genome. Results are analyzed using agarose gel electrophoresis.
*See the addendum in “Resources” for alternative instructions pertaining to the isolation of human DNA using hair.
Instructions, PCR EdvoBeads™ PLUS, Universal DNA Buffer, TE Buffer, Mitochondrial LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube.
All You Need:
DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), (2) Water Baths, Microcentrifuge & Microwave or Hot plate.
Storage:
Some Components Require Freezer Storage Upon Receipt
The Mother of All Experiments: Exploring Human Origin by PCR Amplification of Mitochondrial DNA
In this experiment, students will isolate their mitochondrial DNA and use the Polymerase Chain Reaction (PCR) to amplify two separate regions of the mitochondrial genome. Results are analyzed using agarose gel electrophoresis.
*See the addendum in “Resources” for alternative instructions pertaining to the isolation of human DNA using hair.
Description
PCR Setup – 10 minutes
PCR – 2 hours
Electrophoresis – 60 minutes
Staining – 5 minutes to overnight
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