| Cat. No.: |
335 |
| Group Size: |
For 6 lab groups |
| Time Required: |
PCR Setup – 10 minutes
PCR – 3 hours
Electrophoresis – 60 minutes
Staining – 5 minutes |
| Kit Includes: |
Instructions, RNA LyphoTemplate™, LyphoPrimer™ mix, tubes with RT-PCR EdvoBeads™, RNAse-free water, EdvoQuick™ DNA Ladder, UltraSpec-Agarose™, 50X Electrophoresis Buffer, 10X Gel Loading Solution, SYBR® Safe stain, microcentrifuge & PCR tubes. |
| All You Need: |
DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (3) Water Baths (if performing experiment without a thermal cycler), Microcentrifuge & Microwave or Hot plate. |
| Storage: |
Some Components Require Freezer Storage Upon Receipt |
Reverse Transcription PCR (RT-PCR): The Molecular Biology of HIV Replication
A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory.
Description
PCR – 3 hours
Electrophoresis – 60 minutes
Staining – 5 minutes
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