In this easy PCR experiment, students will make billions of copies of a small amount of DNA in just 90 minutes! They will just need to mix template DNA & primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students will see the increasing amounts of DNA for themselves, taking samples every few cycles and analyzing them on a DNA gel.
Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, DNA Standard Marker, Ultra-pure Water, LyphoTemplate™, TE Buffer, UltraSpec-Agarose™, 50X Electrophoresis Buffer, FlashBlue™ Stain, SYBR® Safe DNA Stain, Microcentrifuge & PCR Tubes.
All You Need:
DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box, (3) Water Baths (if performing experiment without a Thermal Cycler), Microcentrifuge & Microwave or Hot plate.
Storage:
Some Components Require Freezer Storage Upon Receipt
PCR Amplification of DNA
In this easy PCR experiment, students will make billions of copies of a small amount of DNA in just 90 minutes! They will just need to mix template DNA & primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students will see the increasing amounts of DNA for themselves, taking samples every few cycles and analyzing them on a DNA gel.
Description
PCR – 90 minutes
Electrophoresis – 30 minutes
Staining – 5 minutes to overnight
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