Plasmid and lambda DNA are pre-digested with restriction enzymes – endonucleases that recognize and cut double-stranded DNA within or DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.
Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain® Blue Cards, & Disposable Pipets.
All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.
Storage:
Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.
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Mapping of Restriction Sites on Plasmid DNA
Plasmid and lambda DNA are pre-digested with restriction enzymes – endonucleases that recognize and cut double-stranded DNA within or DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.
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