Kit Includes: |
Instructions, PCR EdvoBeads™, universal DNA buffer, TE buffer, EdvoQuick™ DNA ladder, Drosophila LyphoPrimer™, Drosophila WT LyphoTemplate™, potassium acetate, DNA extraction buffer, Proteinase K, Wild-type and White Drosophila, UltraSpec-Agarose™, electrophoresis buffer (50x), SYBR® Safe stain, FlashBlue™ liquid stain, microcentrifuge & PCR tubes. |
Drosophila Genotyping Using PCR
Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.
Description
PCR Setup – 10 minutes
PCR – 2 hours
Electrophoresis – 60 minutes
Staining – 5 minutes to overnight
Related Products
Mystery of the Crooked Cell
Whose DNA Was Left Behind ?
Principles and Practice of Agarose Gel Electrophoresis
Using CRISPR To Treat Cystic Fibrosis