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Drosophila Genotyping Using PCR

Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.

 

 

 

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Description

Cat. No.: 337
Group Size: For 10 lab groups
Time Required: Extraction – 50 minutes
PCR Setup – 10 minutes
PCR – 2 hours
Electrophoresis – 60 minutes
Staining – 5 minutes to overnight
Kit Includes: Instructions, PCR EdvoBeads™, universal DNA buffer, TE buffer, EdvoQuick™ DNA ladder, Drosophila LyphoPrimer™, Drosophila WT LyphoTemplate™, potassium acetate, DNA extraction buffer, Proteinase K, Wild-type and White Drosophila, UltraSpec-Agarose™, electrophoresis buffer (50x), SYBR® Safe stain, FlashBlue™ liquid stain, microcentrifuge & PCR tubes.
All You Need: DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), Water Bath, Microcentrifuge & Microwave or Hot plate.
Storage: Some Components Require Freezer Storage Upon Receipt

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