Alu Human DNA Typing Using PCR

Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet.

*See the addendum in “Resources” for alternative instructions pertaining to the isolation of human DNA using hair.

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Description

Cat. No.: 333
Group Size: For 25 students
Time Required: Extraction – 50 minutes
PCR Setup – 10 minutes
PCR – 90 minutes
Electrophoresis – 60 minutes
Staining – 5 minutes to overnight
Kit Includes: Instructions, PCR EdvoBeads™ PLUS, PV92 LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube.
All You Need: DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, White Light Box (optional), (2) Water Baths, Microcentrifuge & Microwave or Hot plate.
Storage: Some Components Require Freezer Storage Upon Receipt

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