| Kit Includes: |
Instructions, PCR EdvoBeads™ PLUS, PV92 LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube. |
Alu Human DNA Typing Using PCR
Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet.
*See the addendum in “Resources” for alternative instructions pertaining to the isolation of human DNA using hair.
Description
PCR Setup – 10 minutes
PCR – 90 minutes
Electrophoresis – 60 minutes
Staining – 5 minutes to overnight
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